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Sterilizing nutrient mix

To: Aquatic-Plants at actwin_com
Subject: Sterilizing nutrient mix
From: "Shiao Y. Wang" <sywang at whale_st.usm.edu>
Date: Wed, 26 Nov 1997 16:01:29 -0600
References: <199711262048.PAA04119 at acme_actwin.com>

> i am trying to find out information about a sterilization technique for
> alga media (f/2).  i have heard that a chlorination/dechlorination
> process is effective.  is there any information out there about this
> process...particularly which chemicals to buy and how much to add.

I suggest sterilization by filtration using a 0.22 um filter (I use the
disposable ones from Nalgene). The reason is that many nutrient mix
formulations contain B vitamins. The one I use, a modified Guillard's
f/2, contains thiamine, biotin and cyanocobalamin. I would be worried
about a strong oxidizing agent like chlorine denaturing the vitamins.
Same with autoclaving. Filtration with a 0.22 um filter is both safe and
effective.

-- 
Shiao Y. Wang
University of Southern Mississippi
sywang at whale_st.usm.edu

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