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DP Daphnia Magna and Euglena (greenwater) help needed

I just got a starter kit of Daphnia Magna and pure Euglena from LFS Cultures.
I set up two bare tanks inside (one for each culture) as they instructed using
bottled spring water and their Daphnia food. 

                                   DAPHNIA M. QUESTIONS:

Do I need to put a heater in and if so, what temperature should it be kept at?
I keep house at 60 degrees at night and when not at home during the day.  

This late in the year, can I still culture the Daphnia M. outdoors?  If so,
for how much longer? Will a culture survive the winter and thrive next spring?
Outside temperatures for the Indianapolis area this time of year are 40's at
night and 60's to 70's in day.  First killing frost is usually about October
15.  Would I need to aerate outdoor cultures of Daphnia?  What type of
containers and sizes are best for culturing outside?  Should it be in sun or

They gave some instructions on using livestock manure in culture. (The day
after I received the cultures, I came across a pile of horse manure while on a
collecting trip and brought home a bread bag full of it.)  I think I'll start
that solution outdoors ;-).  Any tips on that?  Certainly don't want the odor
in the house.

                              EUGLENA (greenwater) QUESTIONS 

Pretty much same as above:  Do I need to keep it heated inside?  Do I need to
keep it aerated inside before adding Daphnia?  I know if I culture some
outside it will no longer remain pure, but is it too late to start outside and
any tips on doing it outside appreciated.

                                           WATER QUESTION

They suggested that pure spring water was best but I could use old,
unmedicated aquarium water from a healthy tank for both Daphnia and Euglena.
Since Daphnia is sensitive to even small amounts of metal ions, is this really
safe?  I use tap water treated for chlorine, chloramine and copper.  Aren't
the metal ions still there?


Chuck Church
CEFChurch at aol_com   
Indianapolis, Indiana USA

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