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Re: 100% death.

It sounds like some sort of pesticide or other pollutant.  I would set up a
small aquarium (bare) with a few guppies and after a week or two, put in a
handfull of the muck from the bottom of your pond.  Do not use activated
carbon filtration.  If it kills the guppies after a period of time, that looks
like the culprit.