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"Norman D. Edelen, Jr. and Lisa A. Hayashi" <normane at hevanet_com>: NANFA--T. Daniels: shipping fishies




From: Daniels, Todd M. <daniels.todd at mayo_edu>
To: 'Norman D. Edelen, Jr. and Lisa A. Hayashi' <normane at hevanet_com>
Subject: RE: NANFA--R. Rice: RSRICHMOND at aol_com: shipping fishies
Date: Monday, April 28, 1997 6:32 AM

Hi, I read with interest your discussion of the use of Tris. Tris, or 
Trizma Base is shorthand for something like 
Tris(hydroxymethyl)aminomethane. Single people should memorize that: 
it's a good icebreaker on a first date. It is used in research labs as 
a "physiological buffer". You dissolve Tris in low concentration, 
typically 0.01-0.02molar, then add HCl to get the pH to 7.2-7.4. 
Usually salt (sodium chloride, 0.15 molar) is added too. Then it's 
called "tris-buffered saline". 
	Boy, I bet that sounds as pedantic and boring as I thought my 
chemistry professors were 25 years ago! Maybe it's true you become what 
you abhor?
	At any rate, all that chemical jibberish means that this solution

keeps the pH pretty close to "normal" pH for living things like us and 
fish. 
	I read that people commonly use 'salt' to transport fish. A
buffered 
solutions sound like an improvement to me. Do you have the recipe?

----------
From:  Norman D. Edelen, Jr. and Lisa A. Hayashi[SMTP:normane at hevanet_c  
om]
Sent:  Wednesday, April 23, 1997 5:50 PM
To:  B G Granier; David Hall; Todd M. Daniels; Amy Tarlow; Layn 
Luedtke; Vince Brach; Brian Gray; Rob Criswell; Dave Hough; Christopher 
Scharpf; Ray Hunziker; Rick Sharon; John Bondhus; BG Granier; Dave 
Littlehale; Peter Unmack; Garold Sneegas; Jonathon Short; Mark 
Rosenquist; Robert Richmond; Charles Powell; Pat McDonald; Bill Leja; 
Nick LaRocca; Mark Hove; Roger Hockney; Hy Finkelstein; Phil Farrell; 
Norman Edelen, Jr.; Jay DeLong; Steven Corbett; Marcie Ceryes; Robert 
Bock; Robert Rice; Konrad Schmidt; Tom Blanchard; Kate Shaw; Jeff 
Robinson; Eugene Dickerman; Tammy & Keisuke Morimoto; Mike Whitfield; 
Casper Cox; Gerald Binczik; Dean Hendrickson
Subject:  NANFA--R. Rice: RSRICHMOND at aol_com: shipping fishies



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From: robert a rice <robertrice at juno_com>
To: normane at hevanet_com
Subject: RSRICHMOND at aol_com: shipping fishies
Date: Wednesday, April 23, 1997 9:08 AM

Please forward to the list..............


--------- Begin forwarded message ----------
From: RSRICHMOND at aol_com
To: robertrice at juno_com
Subject: shipping fishies
Date: Tue, 22 Apr 1997 23:49:45 -0400 (EDT)
Message-ID: <970422234821_-965295556 at emout12_mail.aol.com>

Hi Robert!

Excellent article!

About thirty years ago when I was transporting fish I used to use TRIS
buffer
- read it somewhere at the time. I transported Fundulus heteroclitus 
and
Cyprinodon variegatus in plastic bags with and without buffer, and 
found
that
the buffered fish survived much better.

The buffer keeps the pH of the water from dropping as its carbon 
dioxide
content increases. This keeps the carbon dioxide as bicarbonate, and it
doesn't cause carbon dioxide narcosis.

I might still have the formulas somewhere. Have you ever heard of using
TRIS
buffer for this?

Bob Richmond
Knoxville TN

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