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Re: KillieTalk Digest V2 #521
In a message dated 11/19/98 3:35:09 PM,
<<
Date: Thu, 19 Nov 1998 10:11:02 -0600
From: William Vannerson <William_Vannerson at ama-assn_org>
Subject: Re: Killifish egg hatching solution recipe sought -Reply
>>Sorry to say my Beginners Guide is also newer and does not contain
the hatching formula you desire. <<
Ed Warner's book, _Success With Killifish_, has a recipe in it. I don't
have a copy here at work but perhaps someone else on-line could check
to see if it's similar to the one you're looking for.
Bill Vannerson>>
Ed Wener's book has the following recipe:
-------------------------------------------------
1 gallon water
1/2 teaspoon non-iodized salt
1 drop Aquari-Sol
5 drops methylene blue
The purpose of the methylene blue is twofold. First, it reduces the light
which is important, because most Killie eggs are light sensitive. Second, if
the eggs absorb the methylene blue and turn blue, discard them. Use a medicine
dropper to remove them. They are either infertile or are fungused. Do not use
more than drops of the solution (Methylene blue) or all the eggs will turn
blue. Methylene blue is not stable, so make up a fresh solution every 3 or 4
days. Methylene blue is not an anti-fungus agent. the Aquari-Sol is used as a
fungicidal agent.
Other dyes like Acriflavine may be used instead of methylene blue, but in
most cases they are too powerful for the beginner to use. Beginners have a
habit of using much too strong a dye solution, so the methylene blue leaves
them a little leeway.
The author recommends plastic Petri dishes to hold eggs and hatching
solution. The dishes may be stacked atop each other cutting down on space. The
dishes should be kept out of light as much as possible and checked daily. Any
eggs which have absorbed the methylene blue should be discarded immediately
using a medicine dropper.
-----------------------------------------------
Who can disagree with Ed Warner's methods?
Lee Harper