Re: [Killietalk] Acriflavine - how much? what concentration?

[Panchax1957 at aol_com]

And I just have to had to add the hatching formula/  hatching solution that's 
worked for me over the years.
:One gallon of fresh tap water, ph  7.5
:Ten drops Methylene Blue
:Five drops Aquarisol
:One Teaspoon salt
note: This formula is for has worked very well for me,  however, it does tend 
to fade in direct light, and over time, becomes less  effective if not fresh.
In a message dated 10/10/2007 4:54:32 P.M. Pacific Daylight Time,  
athensaquatics at juno_com writes:

Hi Guys,  I've got to put my $.02 in.  For me (Ph7,very,very soft) ,2
drops per  gallon is right. 3 drops per seems to retard ( really lengthen)
the  hatching time. My idea is to work ahead but when I draw from this
gallon it  is at 100 % capacity of dissolved air ( oxygen) because of the
constant  aeration ( airstone). Boy does the old saying "cleanliness is
next to  godliness" come into play when working with water incubated eggs.
We ALL  call it "fungus" but it really is bacteria that is attacking the
eggs and  to use "tank water" is ludicrous ( not the most efficient way to
go).  Sincerely, Fred Behrmann
On Wed, 10 Oct 2007 16:35:17 -0400  rfeilzer at aol_com writes:
> I use the same method, a stick and color the  water by eye.? I only 
> use acriflavine and on only a very few of my  pupfish eggs.? 
> Acriflavine is a dye and unless you keep it in the  dark, the 
> pre-mixed solution will fade with time.? In fact, I'm not  sure that 
> darkness will even stop it from fading.? I have one pupfish  species 
> which the eggs are very suceptable to whatever is in the  water and I 
> get a much improved hatch rate, approaching 100% when I  add the 
> acriflavin when the eggs are first picked.? I don't re-add it  when I 
> do water changes on the eggs and yes I do water changes on all  my 
> pupfish eggs.? All the aphyosemions I've ever worked with I have  
> placed the eggs on top of saturated peat in a sealable container,  
> never needed acriflavine with these.? 
> 
> Russ on?the  sunny central coast of Cal.
> 
> 


 



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