# Re: [Killietalk] Acriflavine - how much? what concentration?

```  When making up a premixed supply you will need to remember that in the presents of oxygen methyl blue has a halh life of about 8 hours. I haven't had that experence or seen any data indicating that acriflavine has that "problem".

Gerry> Date: Wed, 10 Oct 2007 13:02:42 -0700> From: jbulterman at yahoo_com> To: killietalk at aka_org> Subject: Re: [Killietalk] Acriflavine - how much? what concentration?> > To be honest, I don't use "drops" as I don't pre-mix a gallon of solution. Instead I use toothpicks, dip one into the bottle of acriflavine and then into the hatching water and the other toothpick into the methyl blue and into the hatching water. The hatching water is about 8 ounces. Using toothpicks allows me to adjust the blue green color with greater precision but use about 50/50 of each. I want the water color light enough to see the eggs developing. The reason I don't pre-mix a gallon of solution is because I raise mainly nothos and SAA's, and not enough Aphys to justify a big pre-mix, otherwise I would. For me the mix works better than each indivdually.> > Joe Bulterman> International School of Choueifat> PO Box 2077> University City Road, Industrial No. 6> Nr. Cricket Stadium> Sharjah, UNITED ARAB EM!
IRATES> +971 6 558 2211> +971 055 942 5049 (Mobil)> > > ----- Original Message ----> From: Thomas Ray <t_a_ray_cissp at yahoo_com>> To: killietalk at aka_org> Sent: Wednesday, October 10, 2007 10:46:33 PM> Subject: Re: [Killietalk] Acriflavine - how much? what concentration?> > Joe: > > Could tell us what amout of each of these is in your mix? For example, > a) how many drops of methyl blue per gallon (or liter)?> b) how many drops of acriflavine per gallon (or liter)?> > Thank you,> --Tom> > Message: 2> Date: Wed, 10 Oct 2007 03:20:17 -0700 (PDT)> From: Joe Bulterman <jbulterman at yahoo_com>> Subject: Re: [Killietalk] Acriflavine - how much? what concentration?> To: killifish discussion list <killietalk at aka_org>> > I usually don't use acriflavine by itself, usually in combo with a> small amount of methyl blue. Mogens had mentioned this combo when I was> trying to figure out to be successful with A. joergensheeli. It worked> for those eggs and I have been using it in combo for all !
non-annual eggs> ever since.> > Joe Bulterman> International School of
Choueifat> PO Box 2077> University City Road, Industrial No. 6> Nr. Cricket Stadium> Sharjah, UNITED ARAB EMIRATES> +971 6 558 2211> +971 055 942 5049 (Mobil) > > > ----- Original Message ----> From: Gary Lasseter <lasseter1113 at yahoo_com>> To: killietalk at aka_org> Sent: Wednesday, October 10, 2007 12:30:47 PM> Subject: Re: [Killietalk] Acriflavine - how much? what concentration?> > Hello again,> > A few peole ask me what concentration of acriflavine I was using in> my water incubation. It is very simple. I take a gallon milk jug full of> de-chlorinated water and add about 2 or 3 drops straight from the> bottle. It doesn't take much at all. In fact it you wanted to you could> probably get away with just a drop.You want your water to be slightly> tinted yellow. One bottle of this stuff will last you a really long time.> Be careful though because anything that you get this stuff on will be> stained yellow, including your fingers!!! > > Later,> Gary Lasseter> > Thomas Ray <t_a_r!
ay_cissp at yahoo_com> wrote:> Gary:> > I've been wondering about using some for my water incubations. > Thanks!> > --Tom> > > > Join the AKA at http://www.aka.org/aka/modules/content/index.php?id=9.> Archives are at http://fins.actwin.com/killietalk/> Modify your subscription at http://www.actwin.com/mailman/listinfo.cgi/killietalk> Join the AKA at http://www.aka.org/aka/modules/content/index.php?id=9.> Archives are at http://fins.actwin.com/killietalk/> Modify your subscription at http://www.actwin.com/mailman/listinfo.cgi/killietalk
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