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Re: [Killietalk] Acriflavine - how much? what concentration?
Hi Guys, I've got to put my $.02 in. For me (Ph7,very,very soft) ,2
drops per gallon is right. 3 drops per seems to retard ( really lengthen)
the hatching time. My idea is to work ahead but when I draw from this
gallon it is at 100 % capacity of dissolved air ( oxygen) because of the
constant aeration ( airstone). Boy does the old saying "cleanliness is
next to godliness" come into play when working with water incubated eggs.
We ALL call it "fungus" but it really is bacteria that is attacking the
eggs and to use "tank water" is ludicrous ( not the most efficient way to
go). Sincerely, Fred Behrmann
On Wed, 10 Oct 2007 16:35:17 -0400 rfeilzer at aol_com writes:
> I use the same method, a stick and color the water by eye.? I only
> use acriflavine and on only a very few of my pupfish eggs.?
> Acriflavine is a dye and unless you keep it in the dark, the
> pre-mixed solution will fade with time.? In fact, I'm not sure that
> darkness will even stop it from fading.? I have one pupfish species
> which the eggs are very suceptable to whatever is in the water and I
> get a much improved hatch rate, approaching 100% when I add the
> acriflavin when the eggs are first picked.? I don't re-add it when I
> do water changes on the eggs and yes I do water changes on all my
> pupfish eggs.? All the aphyosemions I've ever worked with I have
> placed the eggs on top of saturated peat in a sealable container,
> never needed acriflavine with these.?
>
> Russ on?the sunny central coast of Cal.
>
>
> -----Original Message-----
> From: Joe Bulterman <jbulterman at yahoo_com>
> To: killifish discussion list <killietalk at aka_org>
> Sent: Wed, 10 Oct 2007 1:02 pm
> Subject: Re: [Killietalk] Acriflavine - how much? what
> concentration?
>
>
>
> To be honest, I don't use "drops" as I don't pre-mix a gallon of
> solution.
> Instead I use toothpicks, dip one into the bottle of acriflavine and
> then into
> the hatching water and the other toothpick into the methyl blue and
> into the
> hatching water. The hatching water is about 8 ounces. Using
> toothpicks allows
> me to adjust the blue green color with greater precision but use
> about 50/50 of
> each. I want the water color light enough to see the eggs
> developing. The
> reason I don't pre-mix a gallon of solution is because I raise
> mainly nothos and
> SAA's, and not enough Aphys to justify a big pre-mix, otherwise I
> would. For me
> the mix works better than each indivdually.
>
> Joe Bulterman
> International School of Choueifat
> PO Box 2077
> University City Road, Industrial No. 6
> Nr. Cricket Stadium
> Sharjah, UNITED ARAB EMIRATES
> +971 6 558 2211
> +971 055 942 5049 (Mobil)
>
>
> ----- Original Message ----
> From: Thomas Ray <t_a_ray_cissp at yahoo_com>
> To: killietalk at aka_org
> Sent: Wednesday, October 10, 2007 10:46:33 PM
> Subject: Re: [Killietalk] Acriflavine - how much? what
> concentration?
>
> Joe:
>
> Could tell us what amout of each of these is in your mix? For
> example,
> a) how many drops of methyl blue per gallon (or liter)?
> b) how many drops of acriflavine per gallon (or liter)?
>
> Thank you,
> --Tom
>
> Message: 2
> Date: Wed, 10 Oct 2007 03:20:17 -0700 (PDT)
> From: Joe Bulterman <jbulterman at yahoo_com>
> Subject: Re: [Killietalk] Acriflavine - how much? what
> concentration?
> To: killifish discussion list <killietalk at aka_org>
>
> I usually don't use acriflavine by itself, usually in combo with a
> small amount of methyl blue. Mogens had mentioned this combo when I
> was
> trying to figure out to be successful with A. joergensheeli. It
> worked
> for those eggs and I have been using it in combo for all non-annual
> eggs
> ever since.
>
> Joe Bulterman
> International School of Choueifat
> PO Box 2077
> University City Road, Industrial No. 6
> Nr. Cricket Stadium
> Sharjah, UNITED ARAB EMIRATES
> +971 6 558 2211
> +971 055 942 5049 (Mobil)
>
>
> ----- Original Message ----
> From: Gary Lasseter <lasseter1113 at yahoo_com>
> To: killietalk at aka_org
> Sent: Wednesday, October 10, 2007 12:30:47 PM
> Subject: Re: [Killietalk] Acriflavine - how much? what
> concentration?
>
> Hello again,
>
> A few peole ask me what concentration of acriflavine I was using
> in
> my water incubation. It is very simple. I take a gallon milk jug
> full of
> de-chlorinated water and add about 2 or 3 drops straight from the
> bottle. It doesn't take much at all. In fact it you wanted to you
> could
> probably get away with just a drop.You want your water to be
> slightly
> tinted yellow. One bottle of this stuff will last you a really long
> time.
> Be careful though because anything that you get this stuff on will
> be
> stained yellow, including your fingers!!!
>
> Later,
> Gary Lasseter
>
> Thomas Ray <t_a_ray_cissp at yahoo_com> wrote:
> Gary:
>
> I've been wondering about using some for my water incubations.
> Thanks!
>
> --Tom
>
>
>
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>
>
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Sincerely,
Fred Behrmann
Athens Aquatics
37 W. Bridge Street
Catskill, NY 12414 Phone:(518) 943-2630
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