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Re: [Killietalk] daphnia eggs
Joe,
I am getting your message twice, and I do not get other killietalk
messages in duplicate. Perhaps others might comment if they are also
getting them twice. I have checked your killietalk subscription and it
appears to be OK. I wonder if there is something in your mailer setup
that is causing this.
Barry Cooper
Barry J. Cooper
Sweet Home, OR 97386
Joe Bulterman wrote:
> Some years back, while living in Virgina, I decided to see if I could store
> daphnia in the freezer over the winter and then defrost into a living culture
> in the Spring. I collected culture samples (total about 12 ounces of liquid
> and "black peper-like specks from the surface) from both the top and bottom of
> the active daphnia tub and placed in the freezer for 5 months. In the Spring,
> I took the frozen culture and placed it outside in a plastic shoebox. In about
> 3-4 days the frozen culture "hatched" and I had a new thriving culture which
> started my Spring collection. I'm not sure if this is the type of information
> we were seeking but that is my experience with regenerating daphnia in the
> future.
>
> JoeB
>
> --- "J. C." <hillbillynursery at yahoo_com> wrote:
>
>
>> Ok I know they are not eggs but I was not going to
>> try and remember the name and spelling. It happened to
>> come up rescently on the AKA forums. It was one of
>> those weird things as I was going to ask the same
>> thing. I think it was Barry's advice to read something
>> in the library on the site which I did with no real
>> answer. I use daphnia in my fry boxes when the fry
>> reach about a week old. By this time the fry can eat
>> the new born daphnia. I was gone most of the weekend
>> so the tanks stayed dark for the most part. My daphnia
>> cultures are on a bottom shelve and the light never
>> goes off but our frequent short power failures. One of
>> the 3 upper shelf fry boxes daphnia looked weird when
>> I took the time to clean the fry boxes and tanks after
>> the rough weekend(one night spend in the ER with a
>> friend). I fed the tanks and gave all the fry boxes
>> with daphnia a splash of green water like normal from
>> time to time from Thur. through Tues.. Well Yesterday
>> it looked like someone sprinkled black pepper all in
>> the fry box. Most of the daphnia looked normal again.
>> I think it was the dark color from the egg cases that
>> made then look unusual.
>>
>> I want to store these eggs as a back up if the
>> cultures crash. Does anyone have an idea how to store
>> and get these eggs to hatch? Like how long can they be
>> stored before hatching. What triggers have you used to
>> get them to hatch and duration? In the article from
>> the AKA library on live foods they list drying the
>> eggs, cold shock/storage and a few other things but
>> does not give any time frame.
>>
>> Next is how to reproduce the results of getting eggs.
>> Again the article list several things that can work
>> with one of them being daylight length which is what I
>> think triggered this one box as it was the one corner
>> and I never turned the tank lights on just the room
>> light. I know they were not starved into production of
>> eggs as more boxes with daphnia in them would have
>> done this before now as they have went a week without
>> being fed green water. Cold and hot water were also
>> given as reasons.
>>
>> I was sent some eggs from a person on this list
>> which did not ever hatch for me. He sent them on a
>> paper towel and I wet them apon arrival. I am thinking
>> I was missing the incubation triggers like drying
>> Notho. eggs. Maybe they needed either a longer dry
>> spell or a cold spell before hatching them. This is
>> the reason I came to the list as the forums do not get
>> used by all. Hope yall can help.
>>
>> Later, John
>>
>> John Cox of Cumberland Killifish
>> Honey Robber beekeeping and removal services
>>
>> Please join A Fishy World my new email group all
>> about fish at AFishyWorld-subscribe at yahoogroups_com
>>
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>>
>
>
> Joe Bulterman, CPA/CELTA
> Raffles-H.U. International College
> No 2 Teaching Bldg, 3rd Floor, Huizhou University
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>
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