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Re: [Killietalk] daphnia eggs
Some years back, while living in Virgina, I decided to see if I could store
daphnia in the freezer over the winter and then defrost into a living culture
in the Spring. I collected culture samples (total about 12 ounces of liquid
and "black peper-like specks from the surface) from both the top and bottom of
the active daphnia tub and placed in the freezer for 5 months. In the Spring,
I took the frozen culture and placed it outside in a plastic shoebox. In about
3-4 days the frozen culture "hatched" and I had a new thriving culture which
started my Spring collection. I'm not sure if this is the type of information
we were seeking but that is my experience with regenerating daphnia in the
future.
JoeB
--- "J. C." <hillbillynursery at yahoo_com> wrote:
>
> Ok I know they are not eggs but I was not going to
> try and remember the name and spelling. It happened to
> come up rescently on the AKA forums. It was one of
> those weird things as I was going to ask the same
> thing. I think it was Barry's advice to read something
> in the library on the site which I did with no real
> answer. I use daphnia in my fry boxes when the fry
> reach about a week old. By this time the fry can eat
> the new born daphnia. I was gone most of the weekend
> so the tanks stayed dark for the most part. My daphnia
> cultures are on a bottom shelve and the light never
> goes off but our frequent short power failures. One of
> the 3 upper shelf fry boxes daphnia looked weird when
> I took the time to clean the fry boxes and tanks after
> the rough weekend(one night spend in the ER with a
> friend). I fed the tanks and gave all the fry boxes
> with daphnia a splash of green water like normal from
> time to time from Thur. through Tues.. Well Yesterday
> it looked like someone sprinkled black pepper all in
> the fry box. Most of the daphnia looked normal again.
> I think it was the dark color from the egg cases that
> made then look unusual.
>
> I want to store these eggs as a back up if the
> cultures crash. Does anyone have an idea how to store
> and get these eggs to hatch? Like how long can they be
> stored before hatching. What triggers have you used to
> get them to hatch and duration? In the article from
> the AKA library on live foods they list drying the
> eggs, cold shock/storage and a few other things but
> does not give any time frame.
>
> Next is how to reproduce the results of getting eggs.
> Again the article list several things that can work
> with one of them being daylight length which is what I
> think triggered this one box as it was the one corner
> and I never turned the tank lights on just the room
> light. I know they were not starved into production of
> eggs as more boxes with daphnia in them would have
> done this before now as they have went a week without
> being fed green water. Cold and hot water were also
> given as reasons.
>
> I was sent some eggs from a person on this list
> which did not ever hatch for me. He sent them on a
> paper towel and I wet them apon arrival. I am thinking
> I was missing the incubation triggers like drying
> Notho. eggs. Maybe they needed either a longer dry
> spell or a cold spell before hatching them. This is
> the reason I came to the list as the forums do not get
> used by all. Hope yall can help.
>
> Later, John
>
> John Cox of Cumberland Killifish
> Honey Robber beekeeping and removal services
>
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Joe Bulterman, CPA/CELTA
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