Re: [Killietalk] carbonates (probably)

[Chris <cgraseck at optonline_net>]

Bob,
Since the post is on rainforest species (non annuals); is CO2 still a
trigger for enzyme production?

Cheers,
Chris

-----Original Message-----
From: killietalk-bounces+cgraseck=optonline_net at aka.org
[mailto:killietalk-bounces+cgraseck=optonline_net at aka.org] On Behalf Of
rgoldstein at rjgacarolina_com
Sent: Monday, January 02, 2006 9:59 AM
To: killifish discussion list
Subject: Re: [Killietalk] carbonates (probably)

>>
> High GH (8 or10+ degrees) seems to make eggs of rainforest species
have
> trouble hatching. It acts about like adding too much dye and the
chorion
> seems to get too tough to be broken down by the hatch enzymes. I have
> never seen sound documentation of this, but it is common mythology
that
> seems to hold sorta true. IDK what effect it has on the ovaries,
because
> my fish simply won't let me fool with them.
>

First of all, shame on you for trying to fool with your fishes' ovaries
:). But back to the dye interaction with chorions. The internal layers
of
the chorion are proteins subject to destruction by chorionase from the
throats of the hatching fry. I don't know how dyes could affect these
proteins and not the fry's other proteins, which would kill them. I
think
a more likely effect of too much dye (and I agree there can be too much)
is that it prevents bacterial growth necesary to generate the carbon
dioxide needed to stimulate the release of chorionase. No bacterial
metabolism leads to no CO2 trigger, to no release of hatching enzyme,
and
no dissolution of the inner layers of the chorion. Like you, I have no
evidence, but this is just a biologist's thought processes at work (a
rare
event in my case). - RJG


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