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Re: [Killietalk] RE: Challange??



Koran, David HQ02 wrote:

For starters, you probably need to culture "daphnia" in hard water or
have calcium present in order for the animals to develop their exo-skeleton.


Ahh, another oft repeated false assumption. Daphnia presumably do require some calcium in their environment, but the shell, or carapace, is NOT calcified. I quote from http://www.cladocera.fsnet.co.uk/morphology.htm:

"The Cladocera are relatively primitive crustaceans with their bodies completely enclosed by an uncalcified shell (or carapace <http://www.cladocera.fsnet.co.uk/female1.htm>) and having 9 or 10 pairs of appendages <http://www.cladocera.fsnet.co.uk/daphnia.htm> - antennules, antennae, maxillae and mandibles attached to the head, and five or six trunk limbs <http://www.cladocera.fsnet.co.uk/semmorph.htm>, which are using for feeding and respiration".

Personally I have had Moina cultures going for some years now in dead soft water. As the tubs are in the open they are filled mostly with rain water (I'm in Oregon, after all).

People often assume that because biological structures are hard, they are calcified. That is often not the case. Our finger nails, the hooves of horses, cattle, etc., the shell of tortoises and turtles, are not calcified but are composed of compacted keratin (mostly), a protein.

I'm not discouraging people from having some calcium in their culture water, but it is not to allow the Daphnia to form the carapace.

Barry

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