[Killietalk] artemia..again

["Scott Taylor" <staylor at brevardparks_com>]

Not that the subject (artemia) has not been beaten to death, but I am having
an unexplained problem with hatching: have been doing it for years, fairly
uneventfully, but within the last several months I frequently have batches
that fail to hatch at all.  I have changed nothing in my technique: about 20
ppt salinity (aged seawater/aged tap water), vigorous aeration.  I have
changed cyst manufacturer, to no avail: if I start two batches (usually in 2
liter inverted plastic soda bottles), one may fail and the other not.
Sometimes all fail for a couple days in a row and then they do fine.  There
is no pattern that I can detect.  I always rinse containers in hot tap water
between hatchings, sometimes have even tried mild bleach solution as a
rinse/soak.  Any suggestions welcome?
dst

D. Scott Taylor, Ph.D.
Brevard County Environmentally Endangered Lands (EEL) Program
Central Region Land Manager
5560 North US Highway 1
Melbourne, FL 32940
tel: 321.255.4466
FAX: 321.255.4499
email: staylor at brevardparks_com



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