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Re: Lets test it with DAG MON RE: Skewed sex ratios and Androgens (STOP USING ACRIFLAVIN!)




In a message dated 12/19/02 4:58:48 PM, jwwiii at pacbell_net writes:

<< Now, let's postulate that survival, with 

that factor present, depends on either acriflavin (and/or methylene blue), 

or the eggs all die and end up being consumed by fungus.  >>

Yes, the fact that fish and eggs die makes the statistical design approach 
chancy at best. In my experiences, the polymers did not die or disappear so 
all data that was generated resulted in a response. Almost all new technology 
required scouting experiments to establish criteria and then follow up to 
establish quantitative effects. Nevertheless, someone with a couple years to 
work on this in a controlled manner could develop some useful data. Not me, I 
retired from that kind of intensive study to 'relaxed' killifish keeping. 
Besides, if we always got a few more females than males, there be no 
challenges or lonely hearts lists.

Lee Harper
Media, PA 
USA
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