Re: Cryogenic freezing of Killie eggs for species preservation

[Bill Martin <bmartin at gfhnrc_ars.usda.gov>]

While I was working on a gene splicing project about ten years ago,  I came 
across a reference to splicing growth hormone genes into trout 
embryos.  They produced fish that grew to large sizes very quickly ( I 
think some one tried it with SJO in Canada ((that parts a joke)).  We had 
to freeze the freshly spliced mouse embryos in liquid nitrogen for a while 
before letting them develop.  They probably had to do the same thing with 
the trout, so I suspect all the techniques have been developed.  I can't 
see anyone financing a project like this however.  It would take annual 
funding to maintain the cells in liquid nitrogen and an initial large grant 
to start the project, so I doubt that it would ever be done. 

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