RE: Cryogenic freezing of Killie eggs for species preservation

["Tranquility Base" <TranquilityBase at NetZero_Net>]

Hi Bill,

I know of no work regarding the cryogenic preservation of killifish eggs. In
any event, the facilities that would be required for a cryogenic operation
exceed the capacity of most killikeeper's budget.

Peace,

~RJ~



-----Original Message-----
From: owner-killietalk at aka_org [mailto:owner-killietalk at aka_org]On
Behalf Of Bill Wallace
Sent: Tuesday, August 06, 2002 4:08 PM
To: killietalk at aka_org
Subject: Cryogenic freezing of Killie eggs for species preservation


Has anyone tried cryogenic freezing of killie eggs?  Is there any data on
hatch rates after freezing, and/or methodology?  It seems like it would be a
good way to preserve the purity and availability of certain species.  If we
could get a biology student willing to do a Masters or PhD on it, and
perhaps get longer term government funding to run a program, then we would
be able to preserve a great many more species (including non-killi fish).
After the initial research was done, it would likely be possible to have
continuous work done both by volunteers using established guidelines, as
well as in a University setting as part of an icthyology course or as part
of a Master's work in biology to add new species and confirm hatch rates etc
for them.  I'm guessing that it would have to be phrased somewhat
differently to get funding, but I'm fairly certain it would be possible to
get reasonable amounts of money for this.

It might also be possible to flash freeze fry and have them live when they
are thawed as long as the fry are sufficiently small.  I know that insects
can often be frozen and still live, but I don't know what the issues would
be with fry.  Some fishes naturally contain various types of anti-freeze,
which might be a way to help the process.

Bill
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