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RE: Regeneration of Daphnia after Freezing



Thanks for sharing this Joe.  Probably a damn good back up against those
inevitable crashes.  I have always been able to recover from the crash by
doing a 90% water change over the mulm at the bottom, adding a little green
water and waiting a few days.  Having a few bags in the freezer as a back up
would work fine.

Peace
john

On 03-Mar-02 Joseph Bulterman wrote:
> Some time ago, someone wrote in asking if daphnia
> could be spontaeoneously reproduced after freezing
> some of the the bottom barrel contents in a freezer. 
> So, last Fall, from my inside culture of Daphnia
> Moina, I extracted about 4 ounces of whatever was
> collecting on the bottom of my dapnnia culture
> (daphnia, snails, snail droppings, etc.), placed it in
> a plastic fish bag and put it in the freezer.  About a
> week ago, I placed the iceball in a guart of fresh tap
> water and let it sit by a window.  I noticed today
> that I have dancing daphnia in the restarted culture. 
> This of course makes sense that if it freezes ouside
> and comes back, that it will do the same inside but I
> had a few extra minutes and thought I'd try it just
> the same.  Who knows, maybe someone will start selling
> frozen cultures as a starting medium.
> 
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------------------------------------
John N. Alegre                   o
Andante Systems               o
eCommerce Consulting       o
Custom Web Development <*{{{{}><
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E-Mail: listhub at andante_mn.org
Date: 05-Mar-02
Time: 15:43:58
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