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Re: Use of acriflavine
That's interesting Brian because I have had a similar experience.
Some years ago I added too much acriflavine solution to some S. boitonei
eggs that I was water incubating. When the eggs hatched, I also had several
pairs of conjoined twins. One pair lived and swam for a couple of months
and grew to about 1 cm. in length.
Dan Katz
----- Original Message -----
From: "Brian R. Watters" <bwatters at sk_sympatico.ca>
To: <killietalk at aka_org>
Sent: Friday, December 07, 2001 2:07 PM
Subject: Use of acriflavine
> Barry Cooper wrote:
>
> >
> > Where does this information about acriflavine "burning" eggs or
> > bacterial walls come from? Acriflavine is a well-known mutagen
> > that has a bacteriostatic effect due to its ability to cause
> > mutations in the bacteria. It can also cause mutations in fungal,
> > yeast and animal cells. Concerns have been expressed in the past
> > about its possible mutagenic effect on eggs. It is possible that
> > if used in high concentration it could affect the egg (or
> > spematozoa) preventing successful fertilization and/or
> > development of an embryo, although I'm not aware of any direct
> > proof of that.
> >
>
> As you say, there would not appear to be any direct proof of acriflavine
> affecting the development of an embryo. However, I strongly suspect that,
if
> used in very high concentrations, it can.
>
> Many years ago, I had very bad cases of velvet in a couple of populations
of
> wild Nothos from Malawi and I treated these with very heavy doses of
> acriflavine added to the breeding setups. (This in itself was unusual
> because wild Nothos tend to be very much more resistant to velvet that
> aquarium raised specimens). When I hatched eggs spawned in both these
setups
> (from the couple of weeks or so that I was using the heavy medication), I
> got a small proportion (less than one percent) of fry that were siamezed
in
> various ways. I had fry joined at the abdomen, fry with two heads (with a
Y
> shape, the bifurcation being immediately behind the gills), four-eyed fry,
> etc. Incidentally, I have some interesting photomicrographs of these
little
> "monsters". I hatched out large numbers of fry from other spawning groups
at
> the same time, that were not treated with acriflavine, and none of those
> produced any such mutants.
>
> Deformities of this nature could, I suppose, have been the result of other
> factors but the correlation between the occurrence of the siamezed fry and
> the very heavy acriflavine treatment used at the time the eggs were
spawned
> would then have to be an amazing coincidence.
> ___________________________________________
> Brian R. Watters
> University of Regina
> Regina, Sask. S4S 0A2, Canada
> Ph: (306) 584-9161 (home); (306) 585-4663 (work)
> Fax: (306) 585-5433
> E-mail: bwatters at sk_sympatico.ca
>
>
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