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RE: Nothobranchius guentheri
Tyrone,
Where does this information about acriflavine "burning" eggs or bacterial walls come from? Acriflavine is a well-known mutagen that has a bacteriostatic effect due to its ability to cause mutations in the bacteria. It can also cause mutations in fungal, yeast and animal cells. Concerns have been expressed in the past about its possible mutagenic effect on eggs. It is possible that if used in high concentration it could affect the egg (or spematozoa) preventing successful fertilization and/or development of an embryo, although I'm not aware of any direct proof of that.
Barry
At 10:26 AM 12/7/2001 +0200, you wrote:
>On 6 Dec 2001, at 20:50, Tranquility Base wrote:
>
>> But all of the eggs we pick from
>> mops are all already fertilized when they are collected. In the case
>> of Nothos putting the acriflavine in with the parent fish seems to
>> interfere with the fertilization process.
>
>The acriflavine "burns" the egg chorion preventling the
>sperm from fusing with the eggs. In the same way it
>"burns" the bacterial cell walls preventing them from
>latching on to the egg and killing it.
>By playing with the acriflavine concentration you can
>reach a middle ground where the eggs aren't damaged too
>seriously but the bacteria can't bind to the eggs.
>
>I see no need to add acriflavine to the notho tank to
>get good eggs. If the nothos have fertilized the eggs
>they are good and won't just die unless the peat is a
>stinking horrid mess.
>
>If your nothos are giving infertile eggs I would rather
>look at diet and temp. Raise the temp and give a better
>diet. Also, young fish are just slightly less fertile
>than older fish.
>
>As a side note, the peat should adsorb all and any
>acriflavine in the water. It pretty much acts like
>carbon.
>
>Bye
>
>Tyrone Genade
>tyronegenade at yahoo_com
>http://www.tyronegenade.0catch.com
>
>*************************************************************
>P450 Lab, Biochemistry Department
>University of Stellenbosch, 7602, South Africa
>Ph: +27-021-808-5876, fax: +27-021-808-5863
>**********************************************************
>
>"No, the LORD has told us what is good. What He requires
>of us is this: to do what is just, to show constant love,
>and to live in humble fellowship with our GOD."
> Micah 6:8
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___________________________________________________
Barry J. Cooper, Prof., Dept. Biomedical Sciences, Cornell University
Current address: 27505 Riggs Hill Rd.
Sweet Home, OR 97386 (bjc3 at cornell_edu)
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