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killifish and population genetics



Hi all

Lets see if we can't kick of this week with a nice killi 
debate...

Nothos show tremendous variety. If they didn't they 
would become extinct. (Tree that does not bend breaks---
Confucious etc...)

No doubt that in any population there are bad genes. For 
the population of exist and ultimately evolve (not to 
say into a new species but rather a better adpated form 
of the same species) those bad genes have to be kept at 
low levels. Natural selection will cull the population 
to maintain this. To some extent mate selection also 
does its part.

So... the more isolated the population the "stronger" we 
must assume the populations collective genotype to be. 
Which is why we rarely pick up genetic problems with our 
nothos---all the bad genes are at very low frequencies. 
While when we look at less isolated populations the 
picture changes. Guppies and swords are good example. 
Their environments are far less sellective as the fish 
have widers distribution. Bad genes can escape selction 
by moving to new environments were the selection critea 
no longer exists. They may also find themselves in an 
environemnt which selects in favour of a nearby gene (on 
the same chromosomes). Such linked Bad genes genes are 
difficult to get rid of as they afford the individual a 
selective advantage.

Now, we go fishing and we take a few fish from such an 
environment. Lets sya we are lucking and we pull all 
fish which lack that Bad gene. Great! But we can be just 
as unlucky and pull out 1 fish with the trait (in spite 
of the 1% frequency of that allele---the Bad gene for 
our purposes). This 1 fish by some twist of fate lands 
up in say Monty's fishroom where being the great breeder 
he is spawns his fish in great numbers and distributes 
them throughout the AKA. Initialy he started with 6 fish 
of which 1/6 had the bad gene. By the time he is 
finished the bad gene frequency has jumped from 1% to 
17%. Worst of all it may be that Wright has bought a 2 
pr of fish of whihc 1 pr has the Bad gene. Wright being 
the die hard he is perservers with is fish to the point 
were his fish have had it. In the mean time Monty has 
picked up problems as by some chance the females he had 
favoured the males with the Bad gene (becuase it was 
linked to a gene which gave them better and more 
attractive blue spangling---for arguements sake). As it 
stands the frequency of the Bad gene is now 60% and 
everyone is picking up problems now with fertility...

What is the moral of the story? Simply that genetics is 
pot luck. We often can't see the genes that cause 
problems. We tend to select for the visable traits which 
for all we know is linked to some bad gene. Solution? 
Gang breeding? What if the trait is linked to another 
trait which promotes mate selection?

If we look back on the strains which have perservered we 
find the good old AUS, GAR (mine dates back to 1981!), 
whitei and a few nothos. Why were these so successful? 
1) Large populations were set up and crossing out 
regularly occured.
2) High selcection critirea was practiced. Not just 
colour and form but also in fercundaty.
3)some strains just had very little junk. Most nothos 
and Cynolebias are resistant to ibreeding as they have 
had to shed their bad genes long ago to survive.

With many of the imports now days maybe 10 prs comes out 
of the wild. While the allele frequency of the bad genes 
may be 0.1% this doesn't preclude  bad genes coming out 
with those 10 prs. With our new theories on maintaining 
wild population we then set them up for group spawning 
and before we know it (we have no test...) those bad 
genes are everywhere. This is in complete contridiction 
to how killies were bred in the hay day where a multiple 
prs were set up and bred and from that strains were 
fixed.

Perhaps we are doing ourselves in with our breeding 
practices? Your thoughts?

The names Monty and Wright were selected as we all know 
them as damn-good-breeders and killikeepers so we can 
keep this in perspective. Regardless how good a 
killikeeper you are genetics is pot luck and inspite of 
the best intentions, if you get bad stock that is all 
you will ever have...

As a side not, the frequency of cystic fibrosis is 
abnormally high in our current caucasian population. One 
hypothesis for this is that it is linked to a gene or is 
the gene which afforded some protection to the plague 
which swept through Europe. This is just and example of 
inadvertant selection of a bad trait while selecting for 
the good.

Bye

Tyrone Genade
Southern African Killifish Society Coastal & Offshore Coordinator
AKA 08248
tyronegenade at yahoo_com
http://www.geocities.com/tyronegenade

Please excuse my spelling.
I'm too lazy to use my spell checker.
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