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RE: Re[2]: Extending Diapause I in annuals -Inert liquids-
Silicone liquids come in a wide variety of viscosities for use as viscosity
test standards.
-----Original Message-----
From: -RJ- [mailto:TranquilityBase at NetZero_Net]
Sent: Tuesday, June 26, 2001 10:47 AM
To: killietalk at aka_org
Subject: RE: Re[2]: Extending Diapause I in annuals -Inert liquids-
Hi Folks:
Actually I was thinking of something more along the lines of mineral oil or
olive oil. I am not sure if the fluid is inert enough not to harm the eggs
though. And getting it off the eggs would also be a problem. Mercury comes
to mind but it is getting hard to get and is not quite as inert as once
thought.
The characteristics of the liquid would need to be as follows:
Not capable of supporting bacteria.
Not toxic.
Does not contain dissolved oxygen.
Chemically inert.
Readily available.
Nitrogen is a very good idea, even helium or CO2 should work, but is not
readily available in the case of nitrogen and in all cases the gasses are
hard to contain over the long run. As all are clear a leak would not be
noticed until the eggs fungused and died. What we need here is a really
stable unreactive molecule that remains liquid a room temperature.
I can think of lots of inert solids and gasses but my mind draws a blank
when it comes to inert liquids. I suppose that there are not a lot of uses
for a liquid that does nothing. I know that there are a few chemists out
there, and that there is something really obvious I am missing.
Best regards,
-RJ-
-----Original Message-----
From: owner-killietalk at aka_org [mailto:owner-killietalk at aka_org]On
Behalf Of Scott Davis
Sent: Tuesday, June 26, 2001 3:59 AM
To: killietalk at aka_org
Subject: Re: Re[2]: Extending Diapause I in annuals
> I don't think this is what you want to store notho eggs
> in...
Actually that is what they were so enthusiastic about Tyrone. There was a
thread on this list about Jan 1, 1998. (Wow, how time flies!)
One of the killie people (well over 20 years ago) who was really beating the
drum for Ringer's Solution was Art Titus. Art and the Detroit crowd liked
the fact that the solution raised the specific gravity of the holding
solution to that of the eggs internally. They claimed good things for it,
including better hatches. Maybe it was less stressful for the embryo.
Among the benefits they claimed, was that even annual eggs could be better
water incubated (the old 80 degrees plus water incubation). Usually they
used it with water incubating killies
An interesting spin on how it could be used happened when Art incubated some
eggs he received in a trade as "Epiplatys annulatus." In due time they
hatched and grew up to be Nothobranchius guentheri! His opinion of the
sender (who probably assumed they would never hatch and Art would blame
himself for the failure) diminished.
A recipe for it can be found at
http://www-ncmir.ucsd.edu/doc/labmanual/ringer.html
I was startled to note at the bottom of that recipe page, that excess
solution was to be disposed of as a hazardous liquid! He didn't say why.
A Google search (briefly perused) listed several studies of Medaka, carp and
zebra fish where the ringer's solution was used the extend to time in which
eggs could be artificially inseminated. This allowed researchers to study
the process and artificially spawn the fish (well the carp anyway). There
was also a situation involving the study of chicken eggs and another one
where tumors were grown.
The list of hits went on longer that I cared to follow. I'm sure Tyrone
knows about a lot of this anyway.
Killie people obviously are not among the first to use Ringer's solution
with eggs.
Maybe Karl or one of the others who knew Art could add to what they
accomplished using the stuff.
All the best!
Scott
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