[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
how dyes work
Many of the dyes such as Meth.blue, malachite green and
acriflivane (and other acridine dies) you will find in molecular
biology labs where they are used track the movement of DNA or
RNA on gels.
How this is able work is because the dyes intercalate with the
doublstranded DNA and RNA.
This occurs in living systems too. Understandably this would make
life very difficult for microorganism which's sole goal in life is to
divide. The dyes get in the way of the molecules which are tryign to
replicate the DNA or transcribe the code for the various proteins.
The cell essentially burns itself out. The acridine dyes are
especially effective in this regard, even against fungus and other
eukaryotes (such as fish).
Most eukaryotes have nonspecific pumps in there outer
membranes which they use to punp these dies out. Some cells
(such as cancer cells) are very effective int his regard and are
selected for this trait in the presence of these dyes. This is why
the whitespot we have today is so much more difficult to kill with
drugs. The microbes do gain resistance to these dyes. Especially
those dyes which were weak to begin with such as Met.blue.
The strongest and most effective dies are malachite green and the
various acridines. I have had no success with MG (it seems to kill
the egg as well as anything else). The acridine dies work well for
me and as they are specificly directed at bacteria I have to
assume it is them that it kills. The fact that I still get a fluffy egg
now and then would indicate that is doesn't have much effect on
fungus. My fry yields were higher with the antibacterial than the
antifungal I was using.
One of the best products I used was Water lifes Protozin. IT is
directed at fungus and whitespot and other protozoan pathogens.
Isuspect it also has acriflavine or malachite green in along with
The general tonic I use is a mix of 3 acridine dyes and Meth.blue. I
suspect the fact that it is made up of 3 potent dyes and the
oxygenator Meth.blue which makes it so effective as the is very
few bugs (out side hospitables) that can resist 3 drugs. I still
believe that Meth.blue's sole effect is oxygenative.
In regard to tanning the membranes of bacteria etc... This is quite
is likely. Acriflavine definetly has an effect on the membranes such
that you can see it. The tannins and humic acids work in the same
way. They bind to the membrane of the pathogen and target making
is very difficult for the two to merge. If the bactria can't make
contact it can't do any damage (at least the bacteria we do battle
with). The power of Potassium Permangamate is in its highly
oxidative nature. It will literally burn the bacteria's our membrane
(mainly proteins and sugars) away. I won't dismiss the crosslinking
theory in regard to the KMnO4.
Have a happy day!
Department Biochemistry Molecular Cell Physiology Lab
University of Stellenbosch Ph: +27-021-808-5880
Republic of South Africa fax: +27-021-808-5863
"Everything works for good for those who love The Lord"
See http://www.aka.org/AKA/subkillietalk.html to unsubscribe