Re: bam-a-flavine

[George Slusarczuk <yurko at warwick_net>]

Sandy,

Just an addition to my previuos message: If kept in the dark, the 1000
ppm stock solution is reasonably stable.

George 

 
> Hello Sandy,
> 
> I use a 2 ppm of neutral acriflavine solution as the incubating medium.
> Some people use a higher concentration.
> 
> I prepare a 1000 ppm stock solution of acriflavine and dilute it to make
> the 2 ppm working solution. Depending on the accuracy of your balance --
> the more accurate, the smaller amount of stock solution you can prepare
> (to keep it relatively fresh) -- use:
> 
> 1.00 g acriflavine in 1.00 liter = 1000 ppm stock solution
> 100 mg in 100 mL = 1000 ppm, or
> 25 mg in 25 mL = 1000 ppm.
> 
> Then I add 1.0 milliliter of that 1000 ppm stock solution to 0.5 liter
> of incubating water to make a 2 ppm working solution, which I then
> dispense. (To make a 4 ppm working solution you would add 2 mL of the
> stock solution into 0.5 liter of water, etc.) To measure out 1 or 2 mL I
> use a calibrated "baby dropper" sold in drugstores for measuring
> medicines, vitamins, etc.
> 
> Best,
> 
> George
> 
> Sandy1238 at aol_com wrote:
> >
> > Hi,
> > U. S. drugstores as a rule do not sell Acriflavine solutions. There are
> > pharmacies that are set up to primarily compound prescriptions. Check your
> > phone book for one. If you can't find one on your own try calling a local
> > pharmacy and see if they can refer you to one that compounds. Most pharmacies
> > today are too busy to compound and no longer carry the different chemicals.
> > Sad case but that is progress. Anyone out there know about rules in the U. S.
> > about shipping a prepared produce like this?
> > I think I may have some powder and would be interested in proper solution
> > strengths. Any of you lab people know of this produce causing harm as it is a
> > dye and one drop on your skin takes a while to get rid of. Cleanser works
> > best!
> > Regards,
> > Sandy
> > PS I have a balance for making solutions
> > ---------------
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References:

• Re: bam-a-flavine
• From: Sandy1238 at aol_com

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