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Re: bam-a-flavine
Hello Sandy,
I use a 2 ppm of neutral acriflavine solution as the incubating medium.
Some people use a higher concentration.
I prepare a 1000 ppm stock solution of acriflavine and dilute it to make
the 2 ppm working solution. Depending on the accuracy of your balance --
the more accurate, the smaller amount of stock solution you can prepare
(to keep it relatively fresh) -- use:
1.00 g acriflavine in 1.00 liter = 1000 ppm stock solution
100 mg in 100 mL = 1000 ppm, or
25 mg in 25 mL = 1000 ppm.
Then I add 1.0 milliliter of that 1000 ppm stock solution to 0.5 liter
of incubating water to make a 2 ppm working solution, which I then
dispense. (To make a 4 ppm working solution you would add 2 mL of the
stock solution into 0.5 liter of water, etc.) To measure out 1 or 2 mL I
use a calibrated "baby dropper" sold in drugstores for measuring
medicines, vitamins, etc.
Best,
George
Sandy1238 at aol_com wrote:
>
> Hi,
> U. S. drugstores as a rule do not sell Acriflavine solutions. There are
> pharmacies that are set up to primarily compound prescriptions. Check your
> phone book for one. If you can't find one on your own try calling a local
> pharmacy and see if they can refer you to one that compounds. Most pharmacies
> today are too busy to compound and no longer carry the different chemicals.
> Sad case but that is progress. Anyone out there know about rules in the U. S.
> about shipping a prepared produce like this?
> I think I may have some powder and would be interested in proper solution
> strengths. Any of you lab people know of this produce causing harm as it is a
> dye and one drop on your skin takes a while to get rid of. Cleanser works
> best!
> Regards,
> Sandy
> PS I have a balance for making solutions
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