Re: Breeding drosophila

[George Slusarczuk <yurko at warwick_net>]

Hello Daniele,

"Nipagina" = Nipagin, Tegosept, Chemosept, Parasept, Solbrol, is the
ester of para-hydroxy-benzoic acid. Commercialy it is available either
as the ethyl ester, designated as "A" (as in Nipagin A) or methyl ester
(Nipagin M). 

Nipagin M is used as a preservative in FOODS and cosmetics, so I do not
think that it is carcinogenic!

Of course, one can breed Drosophila WITHOUT any mold inhibitor -- the
cultures just do not last as long, one has to subculture more often.
Fresh cultures will increase yield, so it might turn out OK.

Citric acid can lower the pH of the medium too low, so it might have an
inhibiting effect. But many fruits do contain citric (or malic) acid, so
it can not be harmful to the culture.

Best,

George


cerridan wrote:
> 
> Hi friends !!!
> 
> Recently, I have started to breed drosophila (wingless form). I use a simple
> substrate  with corn flakes, oat flakes, sugar, banana, sugar and
> unfermentaitve beer leaven. (The first recipe contained fermentative
> leaven).
> A few breeders have used a chemical preservative called "nipagina" (sorry
> but I'don't know the English translation) to prevent molds. Now, "Nipagina"
> is reteined a cancerogenic substance, so it's a must to use another safe
> anti-mold. Citric acid could be a valid alternative, but I have never tried
> it.
> During this weeks I tried to use unfermentative leaven, because the
> fermentative yeast can produce an excessive substrate swelling. This bulge
> can be stopped with a freezing process. If the substrate is keep at room
> temperature, after two/three days, bulge will be stop. Using a
> unfermentative leaven, bulge is absent and  it's possible to use the
> substrate immediately or after a day. Have anyone tried to use
> unfermentative leaven with good results ??
> By the way, I haven't use any type of preservatized substrate and I have
> equally obtained a good hatch. My  personal experience could  suggest to
> avoid anti-mold substance but I prefer to know your experiences.
> 
> Thank you in advance !
> Daniele Cerri - Italy
> 
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References:

• Breeding drosophila
• From: "cerridan" <cerridan at tin_it>

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