Re: Acriflavin conc.??

[George Slusarczuk <yurko at warwick_net>]

Hello Eric,

I use 2 ppm acriflavine. It gives a distinctly pale yellow solution.

First dissolve the salt in ethanol to make a 1000 ppm stock solution,
then add that to water. Keep both solutions in the dark. 

I use Kodak product #18843, but I got it ages ago, they might have
changed the number! At 2 ppm, 50 g tends to last for quite a while!

Best,

George



Eric Lund wrote:
> 
> Hi folks,
> 
>         One of my coworkers is breeding Cyprinodon variegatus in our
> laboratory and wants to know what concentrations of acriflavin and/or
> malachite green to use for egg incubations.  We have both of these
> compounds as dry crystals so hints on what concentration to make up
> stock solutions and how much to use in the incubation water (or
> molarity of incubation water) would be helpful.
> 
> Thanks,
> 
> Eric Lund, Ph.D.
> Center of Marine Biotechnology
> University of Maryland Biotechnology Institute
> Columbus Center, suite 236
> 701 East Pratt Street
> Baltimore, MD 21202
> 
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References:

• Acriflavin conc.??
• From: Eric Lund <lund at umbi_umd.edu>

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