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Re: Regeneration of Daphnia after Freezing



It is certainly worth a try to freeze bottom siphonings from daphnia
tanks to have as backups  in the event of a catastrophy.

Gary in St. Louis

On Sun, 3 Mar 2002 05:26:48 -0800 (PST) Joseph Bulterman
<jbulterman at yahoo_com> writes:
> Some time ago, someone wrote in asking if daphnia
> could be spontaeoneously reproduced after freezing
> some of the the bottom barrel contents in a freezer. 
> So, last Fall, from my inside culture of Daphnia
> Moina, I extracted about 4 ounces of whatever was
> collecting on the bottom of my dapnnia culture
> (daphnia, snails, snail droppings, etc.), placed it in
> a plastic fish bag and put it in the freezer.  About a
> week ago, I placed the iceball in a guart of fresh tap
> water and let it sit by a window.  I noticed today
> that I have dancing daphnia in the restarted culture. 
> This of course makes sense that if it freezes ouside
> and comes back, that it will do the same inside but I
> had a few extra minutes and thought I'd try it just
> the same.  Who knows, maybe someone will start selling
> frozen cultures as a starting medium.
> 
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