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Re: Decapsulated Brine Shrimp Eggs Storage
During periods when I am away I have decapsulated bs cysts and stored them in the refrigerator, with some success. What I have done is to wash the decapsulated cysts thoroughly by straining through a handkerchief, then allowing as much water as possible to drain out, leaving the cysts as a "paste". I simply transfer that to a jar and refrigerate it. I have to say the hatches aren't as good as when I hatch the decapsulated cysts immediately. It might be worth trying a final wash in salt water (ie hatching water) before allowing to drain and store in the refrig.
I would definitely not incubate them in fresh water. If you are going to make a salt solution to store them in, why not make it saturated?
Something to remember is that the decapsulation process is exothermic. With small quantities of cysts this isn't a problem, but with larger quantities, you need to watch that the temperature doesn't rise too much. You can add ice to the cyst/Chlorox mix to cool it.
Barry
At 11:52 AM 2/17/2002 -0500, you wrote:
>Folks,
>If I decapsulate a one week supply of Brine Shrimp eggs. Do I store them in
>plain water or salt water in the refrigertor?
>I understand that I can store them for up to a month in a brine solution of
>300 gramms of salt per liter of water. I am more concerned with short term
>storage.
>Thanks,
>Joe
>
>
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___________________________________________________
Barry J. Cooper, Prof., Dept. Biomedical Sciences, Cornell University
Current address: 27505 Riggs Hill Rd.
Sweet Home, OR 97386 (bjc3 at cornell_edu)
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