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Seachem
> My SeaChem Fe kit appears to read the reference solution okay, but I'm not
> convinced that it's accurate in the low ranges we're interested in.
It isn't. Somone at Seachem needs to learn A=ecl.
a= absorbance
e= molar extinction coef.
c= concentration
l= path length.
e is something that is a property of the method.
c is what you want to measure
l is what you increase when you want more sensitivity.
it is about that simple.
The "l" is a little short in the spot plates for low range test kits of
any kind.
> When I
> add 5 ml of Tropica MasterGrow per each 50l of aquarium water, I get zero
> Fe readings from the SeaChem kit--which can't be right. If I add enough
> chelated Fe to get a .1 - .2 ppm reading from the SeaChem kit, my tank
> breaks out into a rash of green spot algae (the plants are okay, but I have
> to work extra hard on the plexiglass the next Saturday).
OK, so here is a problem that is going to affect some iron methods. In
some cases, the chrmophore is not strong enough to pull the iron off the
chelating agent.
You can do a potassium persulfate digestion to oxidize the chelating
agent to oblivion, then test the digested matrix. That is the optimal
method for chelated metals.
> FWIW, I'm going to do the same exeriment with the Hach phosphate kit
> (PO-19) vs. the SeaChem phosphate kit.
I've done it, you can't read the seachem test below 0.1 mg/L. This is a
generous assesment, but you may have better eyes. (That is 0.1 mg/L as
PO4 ion, something like 0.03 mg/L P)
I don't know how everyone else feels, but Seachem test kits insult me. I
can look at them and tell they aren't going to do what they say they will
do.
Craig