Re: Books, Isoetes

To: Aquatic-Plants at actwin_com
Subject: Re: Books, Isoetes
From: Elizabeth Worobel <eworobe at cc_UManitoba.CA>
Date: Mon, 18 Mar 1996 19:12:46 -0600 (CST)
Cc: Aquatic-Plants at actwin_com
In-Reply-To: <199603181853.NAA06920 at looney_actwin.com>

George,

Were all cheering from the sidelines ... go ahead, quit your job and your 
other two hobbies and write that plant book ... goodness knows we could 
use a well written 'how to' book.

Paul,

Ive propagated Isoetes successfully in the lab. What I did was to go 
collect some I. echinospora in the fall when the spores were mature. I 
then left them in the fridge for about 3 months. After that time I ripped 
open the micro and mega sporangia (at the base of the leaves) and mixed 
them in a test tube ... within a few weeks you could see many small 
plantlets forming. I was growing them on nutrient agar in test tubes. 
Windelov suggests using peat moss as a substrate instead of agar. Peat 
moss would probably be easier to work with.

dave.